Popular Science articles / 科普文章與專訪
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- 中研院訊 8/24/2023 漫步科研專欄 淺談蛋白質的設計
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Research paper joined the Institute of Biological Chemistry, Academia Sinica in 2018 (Taipei, Taiwan)
* sign means corresponding authorship.
# stands for equal contribution
- Non-catalytic UBL2 domain directs USP11 toward K48-linked polyubiquitin chains
Lee S-R, Chen H-H, Chen R-H*, Wu K-P*
Journal of Biological Chemistry 2025 Dec;301(12):110924.
PubMed PMID: 41207628 - Structural basis of K11/K48-branched ubiquitin chain recognition by the human 26S proteasome
Draczkowski P, Chen S-N, Chen T, Wang Y-S, Shih H-A, Huang JYC, Tsai M-C, Lin S-Y, Lin S, Viner R, Chang Y-C, Wu K-P, Hsu S-TD*
Nature Communications 2025 Oct 15,16,9094
Preprint BioRxiv
PubMed PMID: 41093839 - Elucidating the Mechanism Underlying UBA7•UBE2L6 Disulfide Complex Formation
Chen P-T, Yeh J-Y, Weng J-H, Wu K-P*
eLife 2025,
Preprint BioRxiv - Filamentous chemokine CCL5 structure and the functional aspects
Yuan Y-T, Guo T-C, Wu C-Y, Yeh Y-Q, Wu C-T, Hsu S-Y, Weng Z-W, Ho M-R, Hsu S-TD, Chen Y-C, Chang C-R, Wu K-P*, Jeng U-S*, Sue S-C*
Scientific Reports 2025 Apr 19;15(1):135522.
PubMed PMID: 40253490 - What Strengthens Protein-Protein Interactions: Analysis and Applications of Residue Correlation Networks
Hung TI#, Hsieh Y-J#, Lu W-L, Wu K-P*, Chang CA*
Journal of Molecular Biology. 2023 Dec 15;435(24):168337
BioRxiv preprint
PubMed PMID: 37918563 - PAICS ubiquitination recruits UBAP2 to trigger phase separation for purinosome assembly
Chou M-C, Wang Y-H, Chen F-Y, Kung C-Y, Wu K-P, Kuo J-C, Chan S-J, Cheng M-L, Lin C-Y, Chou Y-C, Ho M-C, Firestine S, Huang J-R, Chen R-H
Molecular Cell. 2023 Nov 16;83(22):4123-4140.e12
PubMed PMID: 37848033 - PTPN23 ubiquitination by WDR4 suppresses EGFR and c-MET degradation to define a lung cancer therapeutic target
Singh S, Yeat NY, Wang Y-T, Lin S-Y, Kuo I-Y, Wu K-P, Wang W-J, Wang W-C, Su W-C, Wang Y-C, Chen R-H
Cell Death & Disease 2023 Oct 11;14(10):671
PubMed PMID: 37821451 - Structural basis of transcriptional activation by the OmpR/PhoB-family response regulator PmrA
Lou Y-C*, Huang H-Y, Yeh H-H, Chiang W-H, Chen C*, Wu K-P*
Nucleic Acids Research. 2023 Oct 13;51(18):10049-10058,
BioRxiv preprint
PubMed PMID: 37665001, PDB: 8JO2, EMDB: 36453 - Robust design of effective allosteric activators for Rsp5 E3 ligase using the machine-learning tool ProteinMPNN
Kao H-W, Lu W-L, Ho M-R, Lin Y-F, Hsieh Y-J, Ko T-P, Hsu S-T D, Wu K-P*
ACS Synthetic Biology 2023 Aug 18;12(8):2310-2319,
PubMed PMID 37556858:, PDB: 8J0A - TRABID inhibition activates cGAS/STING-mediated anti-tumor immunity through mitosis and autophagy dysregulation
Chen Y-H, Chen H-H, Wang W-J, Chen H-Y, Huang W-S, Kao C-H, Lee S-R, Yeat NY, Yan R-L, Chan S-J, Wu K-P, Chen R-H
Nature Communications 2023 May 26;14(1):3050,
PubMed PMID: 37237031 - Cryo-EM reveals the structure and dynamics of a 723-residue malate synthase G
Ho M-R#, Wu Y-M#, Lu Y-C#, Ko T-P, Wu K-P*
Journal of Structural Biology 2023 Jun;215(2):107958 BioRxiv preprint,
PubMed PMID: 36997036,
PDB: 7YQM, 7YQN, EMDB: 34029, 34030, EMPIAR: 11167 - 2.2 Å cryo-EM tetra-protofilament structure of the hamster prion 108−144 fibril reveals an ordered water channel in the center
Chen E. H-L#, Kao H-W#, Lee C-H#, Huang JYC, Wu K-P*, Chen RPY*
Journal of the American Chemical Society, 2022 Aug 3;144(30):13888-13894.
PubMed PMID: 35857020, PDB: 7YAT, EMDB: 33719 - Tumor suppressor BAP1 nuclear import is governed by transportin-1
Yang T-J, Li T-N, Huang R-S, Pan M Y-C, Lin S-Y, Lin S, Wu K-P, Wang H-C L,Hsu S-T D
Journal of Cell Biology 2022,Jun 6;221(6):e202201094,
PubMed: PMID: 35446349 - Structural basis for the helical filament formation of Escherichia coli glutamine synthetase
Huang P-C, Chen S-K, Chiang W-H, Ho M-R, Wu K-P*
Protein Science. 2022 May;31(5):e4304,
PubMed PMID: 35481643, PDB: 7W85, EMDB: 32352 - Identification of disease-linked hyperactivating mutations in UBE3A through large-scale functional variant analysis
Weston KP, Gao X, Zhao J, Kim K-S, Maloney SE, Gotoff J, Parikh S, Leu Y-C, Wu K-P, Shinawi M, Steimel JP, Harrison JS, Yi JJ
Nature Communications 2021 12, Article number: 6809,
PubMed PMID: 34815418 - Simeprevir Potently Suppresses SARS-CoV-2 Replication and Synergizes with Remdesivir
Lo HS, Hui PY, Lai HM, He X, Khan KS, Kaur S, Huang J, Li Z, Chan AKN, Cheung HHY, Ng KC, Ho JCW, Chen YW, Ma B, Cheung PMH, Shin D, Wang K, Lee M-H, Selisko B, Eydouc C, Guillemot J-C, Canard B, Wu K-P, Liang P-H, Dikic I, Zuo Z, Chan FKL, Hui DSC, Mok VCT, Wong K-B, Mok CKP, Ko H, Aik WS, Chan MCW, Ng W-L
ACS Central Science, 2021,May 26; 7(5): 792–802,
PubMed PMID: 34075346 - Direct Visualization of a 26 kDa Protein by Cryo-Electron Microscopy Aided by a Small Scaffold Protein
Chiu Y-H , Ko K-T, Yang T-J, Wu K-P, Ho M-R, Draczkowski P, Hsu S-T D.
Biochemistry. 2021 Apr 13;60(14):1075-1079,
PubMed PMID: 33719392 - VPS34 K29/K48 branched ubiquitination governed by UBE3C and TRABID regulates autophagy, proteostasis and liver metabolism
Chen Y-H, Huang T-Y, Lin Y-T, Lin S-Y, Li W-H, Hsiao H-J, Yan R-L, Tang H-W, Shen Z-Q, Chen G-C, Wu K-P, Tsai T-F, Chen R-H.
Nature communications 2021, 12, Article number: 1322,
PubMed PMID 33637724 - Branched Ubiquitination: Detection Methods, Biological Functions and Chemical Synthesis
Wang Y-S*, Wu K-P*, Jiang H-K, Kurkute P, Chen R-H*
Molecules (Review), 2020 Nov 9;25(21):5200,
PubMED PMID: 33182242 - Insights Into Dynamics of Inhibitor and Ubiquitin-Like Protein Binding in SARS-CoV-2 Papain-Like Protease
Bosken YK, Cholko T, Lou Y-C, Wu K-P, Chang C-A A.
Frontiers in Molecular Bioscience. 2020, Aug 4;7:174,
PubMed PMID: 32850963 - Cryo-EM analysis of a feline coronavirus spike protein reveals a unique structure and camouflaging glycans
Yang T-J, Chang Y-C, Ko T-P, Draczkowski P, Chien Y-C, Chang Y-C, Wu K-P, Khoo K-H, Chang H-W, Hsu S-T D
PNAS, 2020 January 21, 117 (3) 1438-1446
PubMed PMID: 31900356
Research papers at St Jude Children’s Research Hospital (2012-2017) (Memphis, Tennessee, US)
- Insights into links between autophagy and the ubiquitin system from the structure of LC3B bound to the LIR motif from the E3 ligase NEDD4.
Qiu Y, Zheng Y, Wu K-P, Schulman BA.
Protein Science. 2017 Aug;26(8):1674-1680
PubMed PMID: 28470758 - Deubiqutinase activity is required for the proteasomal degradation of misfolded cytosolic proteins upon heat-stress.
Fang NN, Zhu M, Rose A, Wu K-P, Mayor T.
Nature Communications. 2016 Oct 4;7:12907
PubMed PMID: 27698423 - Dual RING E3 Architectures Regulate Multiubiquitination and Ubiquitin Chain Elongation by APC/C.
Brown NG, VanderLinden R, Watson ER, Weissmann F, Ordureau A, Wu K-P, Zhang W, Yu S, Mercredi PY, Harrison JS, Davidson IF, Qiao R, Lu Y, Dube P, Brunner MR, Grace CRR, Miller DJ, Haselbach D, Jarvis MA, Yamaguchi M, Yanishevski D, Petzold G, Sidhu SS, Kuhlman B, Kirschner MW, Harper JW, Peters J-M, Stark H, Schulman BA.
Cell. 2016 Jun 2;165(6):1440-1453
PubMed PMID: 27259151 - A cascading activity-based probe sequentially targets E1–E2–E3 ubiquitin enzymes.
Mulder MPC, Witting K, Pruneda JN, Berlin I, Wu K-P, Merkx R, Neefjes J, Schulman BA, Komander D, Oualid FE, Ovaa H.
Nature Chemical Biology. 2016 Jul;12(7):523-30
PubMed PMID: 27182664 - System-wide modulation of HECT E3 ligases with selective ubiquitin variant probes.
Zhang W#, Wu K-P#, Sartori M, Kamadurai HB, Ordureau A, Jiang C, Mercredi PY, Murchie R, Hu J, Persaud A, Mukherjee M, Li N, Doye A, Walker JR, Sheng Y, Hao Z, Li Y, Brown KB, Lemichez E, Chen J, Tong Y, Harper JW, Rotin D, Moffat J, Schulman BA, Sidhu SS.
Molecular Cell. 2016 Apr 7;62(1):121-36.
PubMed PMID: 26949039#: Equally contributed authors - ITCH WW domains inhibits E3 ubiquitin ligase activity by blocking E2-E3 transthiolation.
Riling C, Kamadurai HB, Kumar S, O’Leary CE, Wu K-P, Marino EE, Ying M, Schulman BA, Oliver PM.
Journal of Biological Chemistry. 2015 Sep 25;290(39):23875-87.
PubMed PMID: 26245901
Research papers at Rutgers University (New Brunswick, New Jersey, US)
- Unveiling transient protein-protein interactions that modulate inhibition of alpha-synuclein aggregation by beta-synuclein, a pre-synaptic protein that co-localizes with alpha-synuclein
Janowska M, Wu K-P, Baum J.
Scientific Reports. 2015 Oct 19;5:15164.
PubMed PMID: 26477939 - ACA-specific RNA sequence recognition is acquired via the loop 2 region of MazF mRNA interferase.
Park JH, Yoshizumi S, Yoshihiro Y, Wu K-P, Inouye M.
Proteins. 2013 May;81(5):874-83
PubMed PMID: 23280569 - Segmental isotope labeling of proteins for NMR structural study using a protein S tag for higher expression and solubility.
Kobayashi H, Swapna GV, Wu K-P, Afinogenova Y, Conover K, Mao B, Montelione GT, Inouye M
Journal of Biomolecular NMR. 2012 Apr;52(4):303-13.
PubMed PMID: 22389115 - Fast hydrogen exchange affects 15N relaxation measurements in intrinsically disordered proteins.
Kim S, Wu K-P, Baum J.
Journal of Biomolecular NMR. 2013 Mar;55(3):249-56.
PubMed PMID: 23314729 - YeeU enhances the bundling of cytoskeletal polymers of MreB and FtsZ, antagonizing the CbtA (YeeV) toxicity in Escherichia coli.
Masuda H, Tan Q, Awano N, Wu K-P, Inouye M.
Molecular Microbiology. 2012 Jun;84(5):979-89.
PubMed PMID: 22515815 - Investigation of the polymeric properties of alpha-synuclein and comparison with NMR experiments: a replica exchange molecular dynamics study.
Narayanan C, Weinstock DS, Wu K-P, Baum J, Levy RM.
Journal of Chemical Theory and Computation. 2012 Oct 9;8(10):3929-3942.
PubMed PMID: 23162382 - The A53T mutation is key in defining the differences in the aggregation kinetics of human and mouse alpha-synuclein.
Kang L, Wu K-P, Vendruscolo M, Baum J.
Journal of the American Chemical Society. 2011 Aug 31;133(34):13465-70.
PubMed PMID: 21721555 - Backbone assignments and dynamics of alpha-synuclein in viscous 2 M glucose solution.
Wu K-P, Baum J.
Biomolecular NMR Assignment. 2011 Apr;5(1):43-6.
PubMed PMID: 20872101 - Detection of transient interchain interactions in the intrinsically disordered protein alpha-synuclein by NMR paramagnetic relaxation enhancement.
Wu K-P, Baum J.
Journal of the American Chemical Society 2010 Apr 28;132(16):5546-7.
PubMed PMID: 20359221 - Structural reorganization of alpha- synuclein at low pH observed by NMR and REMD simulation.
Wu K-P, Weinstock DS, Narayanan C, Levy RM, Baum J.
Journal of Molecular Biology. 2009 Aug 28;391(4):784-96.
PubMed PMID: 19576220 - Backbone NMR assignments of DFP-inhibited mature subtilisin E
Cheng Y-J, Wu K-P, Kim S, Falzon L, Inouye M, Baum J
Biomolecular NMR Assignments 2008 Dec;2(2):131-3.
PubMed PMID: 19636887 - Characterization of conformational ensemble of natively unfolded human and mouse alpha-synuclein: implication for aggregation.
Wu K-P, Kim S, Fela DA, Baum J.
Journal of Molecular Biology. 2008 May 16;378(5):1104-15.
PubMed PMID: 18423664 - Distinguishing among structural ensembles of the GB1 peptide: REMD simulations and NMR experiments.
Weinstock DS, Narayanan C, Felts AK, Andrec M, Levy RM, Wu K-P, Baum J.
Journal of the American Chemical Society. 2007 Apr 25;129(16):4858-9.
PubMed PMID: 17402734
Research papers in Taiwan (2000-2004)
- Novel solution structure of porcine beta-microseminoprotein.
Wang I, Lou YC, Wu K-P, Wu SH, Chang WC, Chen C.
Journal of Molecular Biology. 2005 Mar 4;346(4):1071-82.
PubMed PMID: 15701518 - Letter to editor: 1H, 13C and 15N assignments and secondary structure of mourine angiogenesis 4.
Pan YR, Wu K-P, Lou YC, Liao YD, Chen C.
Journal of Biomolecular NMR (2005), 31, 175-179
PubMed PMID: 15701518 - Structural basis of a Flavivirus recognized by its neutralizing antibody: Solution structure of the domain III of the Japanese Encephalitis virus envelope protein.
Wu K-P, Wu CW, Tsao YP, Kuo TW, Lou YC, Lin CW, Wu SC, Cheng JW.
Journal of Biological Chemistry. 2003 Nov 14;278(46):46007-13.
PubMed PMID: 12952958
KPWu's group research site 