CryoEM Reveals Mechanism of Polymyxin-resistant PmrA-bound Transcription Activation Complex

The cryoEM structure composed of a dimeric PmrA, double stranded DNA, RNA polymerase and cofactor sigma70 was determined and the work is published in Nucleic Acids Research (2023-Sept).

AI-designed Ubiquitin Variant acts as an effective modulator of Rsp5 E3 ligase

We demonstrated the crystal structure of a synthetic ubiquitin variant named R4 (PDB 8J0A) designed by an AI tool ProteinMPNN is identical to wild type ubiquitin. The R4 is an effective allosteric activator of Rsp5 E3 ligase. Work published in ACS Synthetic Biology.

Cryo-EM reveals high-quality structure and conformational dynamics of a 723-aa Malate Synthase G (MSG)

Cryo-EM structure of 723-aa malate synthase G at 2.9 Å (PDB ID 7YQM) and the 1.6 Å X-ray crystal structure of malate synthase G (PDB ID 7YQN), published in Journal of Structural Biology.

The mysterious filament of Ecoli glutamine synthetase decoded by cryoEM

The assembly of filamentous structure of E coli glutamine synthetase, published in Protein Science. PDB ID = 7W85, EMDB entry = 32352.

Papain-like protein of SARS-CoV-2 (C111S mutant), PDB ID = 7D47